# Advanced-service validation

These fixtures extend the pinned public MAGeCK/Yusa excerpt into repeatable
validation for the retained Advanced services.

| Service | Dataset | Oracle |
| --- | --- | --- |
| Demultiplexing | Real Yusa read bodies with controlled terminal variants of two real guide barcodes | Assignment totals at every mismatch allowance from zero through five |
| Distance clustering | All 64 unchanged Yusa guide sequences | Independent ordered two-base word-pair calculation for all 2,016 guide pairs |
| STARS | All unchanged plasmid and ESC counts, placed into explicit validation groups | Independent normalization, difference, rank, binomial score, and score-rank table |
| Advanced Alignment | The unchanged public FASTQs and guide library in `../MageckYusaExcerpt` | Its independently extracted 128-row expected count table |

The per-service READMEs record derivation details and SHA-256 hashes. Controlled
derivatives are functional fixtures, not new biological claims. The Advanced
Alignment test uses the public records without sequence changes.
